Thesis On Thermophiles

Thesis On Thermophiles-77
strains exhibited both higher thermal stability and tolerance to acidic conditions.The study highlights the vast potential of an untapped diversity of thermophilic fungi in the tropics., and only a small number of fungal species thrive at such high temperatures.

strains exhibited both higher thermal stability and tolerance to acidic conditions.The study highlights the vast potential of an untapped diversity of thermophilic fungi in the tropics., and only a small number of fungal species thrive at such high temperatures.Characterisation of this culture indicated the presence of two organisms essential for efficient growth under air: a typical S.

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A range of genes encoding lignocellulolytic enzymes in the acidophilic fungus Acidomyces acidothermus MEY-1 has been cloned and expressed.

The enzymes were found to be thermotolerant and functional under acidic conditions.

Strain ICP appeared to possess an inducible, high affinity transport system for carbon dioxide during growth under air (unlike any previously studied moderate thermophiles), but extensive oxidation of ferrous iron was not achieved even at enhanced carbon dioxide levels.

This lack of oxidation appeared to be the result of autotrophically-growth strain ICP having an apparent higher affinity for the end-product of iron oxidation, ferric iron (Ki 0.4 m M), than the substrate, ferrous iron (Km 0.5 m M).

Maximum growth temperatures of the strains were in the range 50 °C to 60 °C.

Sequencing of the internal transcribed spacer (ITS) regions indicated that 78 fungi belonged to 12 species of Ascomycota and 3 species of Zygomycota, while no fungus of Basidiomycota was detected.Earlier reports of thermophilic fungi were the result of accidental contamination of organic materials incubated at elevated temperatures.These include the isolation of Mucor pusillus (=Rhizomucor pusillus) from bread in 1886, and Humicola lanuginosa (=Thermomyces lanuginosus) from potato slices in 1899 (from Johri et al..Thermophilic fungi can represent a rich source of industrially relevant enzymes.Here, 105 fungal strains capable of growing at 50 °C and p H 2.0 were isolated from compost and decaying plant matter.There is no consensus on the demarcation between the two groups, but typically, a fungus that has a thermal maximum near 50 °C and a minimum below 20 °C is regarded as thermotolerant, while those that grow at 50 °C or above, but not grow at 20 °C or above are regarded as thermophilic.In the present study, the cultivation temperature was maintained at 50 °C during initial screening for thermophilic fungi.Among the estimated 3.0 million fungal species existing in nature, and approximately 100 000 species described, only about 50 species have been found to be able to grow at 50–60 °C.Fungi capable of growing at elevated temperatures are classified into thermophilic and thermotolerant groups.Only when a mixed culture of strain ICP and strain ICH was grown did extensive oxidation occur.A comparative mineral leaching study, with a mesophilic, a moderately thermophilic, and an extremely thermophilic culture indicated that the moderately thermophilic culture was the most robust during the dissolution of a range of minerals.

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